狠狠综合亚洲综合亚洲色丨国产免费久久精品国产传媒丨天天爽夜夜爽夜夜爽精品视频丨无码av天堂一区二区三区丨av激情亚洲男人的天堂

【文獻(xiàn)標(biāo)題】Effects of rearing systems on reproductive hormones secretion and their receptors gene expression in Xianju chickens under summer conditions

【作者】Z. Z. Yin，X. Y. Dong，H. Y. Cao，et.al

【作者單位】南京農(nóng)業(yè)大學(xué)（Nanjing Agricultural University）

【文獻(xiàn)中引用產(chǎn)品】

雞促卵泡生長激素（FSH）ELISA試劑盒

雞孕酮（P4）ELISA試劑盒

雞促黃體激素（LH）ELISA試劑盒

雞催乳素（PRL）ELISA試劑盒

【關(guān)鍵詞】Xianju chicken, reproductive hormone, gene expression, rearing system, summer conditions

【DOI】dx.doi.org/10.3382/ps/pey194

【影響因子(IF)】2.23

【出版期刊】《Poultry Science》

【產(chǎn)品原文引用】

Blood sampling and analysis

At the end of wk 29, 12 h after feed withdrawal, 2 birds were randomly selected from each replicate and blood samples were collected from the axillary vein.Serum was isolated by centrifugation of blood at 3000×g for 10 min, and was stored in 1.5-mL Eppendorf tubes at −70?C until analyses. Serum FSH, P4, LH, and PRL levels were analyzed using RIA kits (Hengyuan Biological Technology Co., Ltd, Shanghai, China) by radio immunoassay, according to the recommendations of the manufacturer. For measurement of FSH concentration, the assay sensitivity, measuring range, intra-and interassay CVs were 0.4 mIU/mL, 1.5 mIU/mL to 100mIU/mL, <10% and <15%, respectively. For measurement of P4 concentration, the assay sensitivity was 0.2 ng/mL, measuring range was 0.5 to 80 ng/mL, and the intra- and interassay CVs were <10%. For measurement of LH concentration, the assay sensitivity, measuring range, intra- and interassay CVs were 0.02, 0.5 to 20 ng/mL, <10% and <15%, respectively. For measurement of PRL concentration, the assay sensitivity was 0.5 ng/mL, measuring range was 5.0 to 100 ng/mL,and the intra- and interassay CVs were <10%. All the samples were measured in one single assay.

完整版PDF文獻(xiàn)請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員！

更多公司福利請關(guān)注“恒遠(yuǎn)生物”VX公眾號！